Coding

Part:BBa_K3697000:Design

Designed by: Christopher Neimeth, Lauren Ramlan   Group: iGEM20_Stanford   (2020-10-22)


mCherry BSU


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 715
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence had been optimized previously for expression in B. subtilis, but when incorporating this sequence into the system we designed, we had to be cognizant of what RBS and promoter we should use in order to get the level of expression we wanted.


Source

Our team originally got this part from the Bacillus Genomic Stock Center (http://www.bgsc.org/) and a plasmid they use regularly for a variety of experiments.

References